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KMID : 1189420100230020057
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2010 Volume.23 No. 2 p.57 ~ p.68
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Effects of Lithospermum erythrorhizon extracts on P. acnes induced cytokine gene expression in human monocytes
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Seo Min-Su
Kim Kyung-Jun
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Abstract
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Objective : This study was performed to evaluate the effect of Lithospermum erythrorhizon extracts on the inflammatory cytokines gene expression by the bacteria of Propionibacterium acnes (P. acnes) which elicits acne in human monocytes, THP-1 cell line.
Experiment : Cytotoxicity of Lithospermum erythrorhizon extracts was analyzed by XTT assay. Real time
RT-PCR was applied to analyze the cytokines gene expressions of IL-8, MCP-1 and TNF-α. Translocation of btranscription factor NF-κB from cytoplasm into nucleus was observed using immunocytochemistry and confocal microscopy.
Results : Lithospermum erythrorhizon extracts did not show cytotoxicity as high as in 1,000 ㎍/㎖ of concentration. Transcription levels of inflammatory cytokines, IL-8, MCP-1 and TNF-α were increased by P.acnes in THP-1 and Lithospermum erythrorhizon extracts decreased the upregulated transcription levels. Lithospermum erythrorhizon extracts significantly inhibited the translocation of NF-κB into nucleus by P. acnes.
Conclusion : This study suggests that Lithospermum erythrorhizon extracts have anti-inflammatory effects
on P. acnes treated THP-1 as decreasing the mRNA expressions of IL-8, MCP-1 and TNF-α. This anti-inflammatory effect of Lithospermum erythrorhizon extracts may be useful in therapeutic treatments for acne vulgaris.
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KEYWORD
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Propionibacterium acnes, acne vulgaris, human monocytes, anti-inflammatory effects, Lithospermum erythrorhizon
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